Abstract
Objective
To evaluate the efficacy and mechanistic bases of the intravenous injection of arsenic trioxide at clinical-relevant doses for treating an imiquimod-induced psoriasis-like mouse model.
Methods
After inducing psoriasis-like skin lesions on the back of mice with imiquimod, mice in each group were injected with a clinical dose of arsenic trioxide through the tail vein. The changes in the gene expression, protein expression and distribution of relevant inflammatory factors were evaluated in the inflicted skin area, for mechanisms underlying the efficacy of intravenous As2O3 intervention. HaCaT cells were used to establish an in vitro psoriasis model and pcDNA3.1-NF-κB overexpression plasmid was transfected into cells to overexpress P65, which further confirmed the role of the NF-κB signaling pathway in the effectiveness of As2O3.
Results
Clinical dose of As2O3 can significantly improve abnormal symptoms and pathological changes in psoriasis-like skin lesions induced by IMQ in mice. While IMQ induced abnormal expression and distribution of inflammatory factors in the RIG-I pathway and the microRNA-31 (miR-31) pathway in psoriatic skin tissues, intravenous As2O3 can effectively regulate and restore the normality. The leading role of NF-κB signaling was evidenced in vivo and validated in vitro using the NF-κB-overexpressed HaCaT cell model.
Conclusion
Clinical dosage of As2O3 may achieve effective treatment of IMQ-induced psoriatic skin lesions by modulating the NF-κB signaling pathway which regulates both the RIG-I and the miR-31 lines of action. Our data provided strong evidence supporting the claim that systemic As2O3 administration of clinical doses can be a promising treatment for psoriasis patients.
Acknowledgment
The authors are grateful to Professor Jinsong Yan, M.D., PhD., at the Department of Hematology, The Second Hospital of Dalian Medical University, for his clinical observations which inspired this research. The authors also owe gratitude to Dr. Ying Liu, at the Department of Dermatology, The First Hospital of Dalian Medical University, for a kind gift of the HaCaT cells. We appreciate the technical support from the Key Laboratory of Specific Pathogen Free Animal of Liaoning Province, Dalian Medical University.
Author contributions
Xiaoji Hao conducted the research and drafted the manuscript. Xiaohui Liu was tasked with an instructive role during research process and aided in data analysis. Shunfei Yu played a critical role during manuscript preparation and provided many valuable feedback and comments. Chang Qin, Ruonan Wang and Chunna Li helped perform the analysis with constructive discussions. Jing Shao (corresponding author) supervised project planning and designing, experimental development, and writing and revision of the manuscript. All authors read the manuscript and agreed with this submission.
Disclosure statement
The authors declare no potential conflicts of interest.
Data availability statement
Please contact the authors for data requests.