Dynamic bacterial communities on reverse-osmosis membranes in a full-scale desalination plant

Abstract

To better understand biofouling of seawater reverse osmosis (SWRO) membranes, bacterial diversity was characterized in the intake water, in subsequently pretreated water and on SWRO membranes from a full-scale desalination plant (FSDP) during a 9 month period. 16S rRNA gene fingerprinting and sequencing revealed that bacterial communities in the water samples and on the SWRO membranes were very different. For the different sampling dates, the bacterial diversity of the active and the total bacterial fractions of the water samples remained relatively stable over the sampling period whereas the bacterial community structure on the four SWRO membrane samples was significantly different. The richness and evenness of the SWRO membrane bacterial communities increased with usage time with an increase in the Shannon diversity index of 2.2 to 3.7. In the oldest SWRO membrane (330 days), no single operational taxonomic unit (OTU) dominated and the majority of the OTUs fell into the Alphaproteobacteria or the Planctomycetes. In striking contrast, a Betaproteobacteria OTU affiliated to the genus Ideonella was dominant and exclusively found in the membrane used for the shortest time (10 days). This suggests that bacteria belonging to this genus could be one of the primary colonizers of the SWRO membrane. Knowledge of the dominant bacterial species on SWRO membranes and their dynamics should help guide culture studies for physiological characterization of biofilm forming species.

Keywords:

• bacterial diversity
• biofilm
• reverse osmosis membrane
• phylogenetic analysis
• culture-independent techniques

Acknowledgements

This work was financially supported by the EU-MEDINA project (Membrane-based desalination – An integrated approach, project number: 036997). The authors acknowledge the AR-VW team who coordinated and performed the sampling and shipment of the material used in this study, which was part of the FP6 framework European project, MEDINA. The authors wish to thank M. Suzuki and P. Galand for their critical reading of the manuscript and the anonymous reviewers for their critical comments, which helped to improve the manuscript quality.