Adsorption of HSA, IgG and laminin-1 on model hydroxyapatite surfaces – effects of surface characteristics

Abstract

Ellipsometry and mechanically assisted sodium dodecyl sulphate elution was utilized to study the adsorption of human serum albumin (HSA), human immunoglobulin G (IgG), and laminin-1, as well as competitive adsorption from a mixture of these proteins on spin-coated and sintered hydroxyapatite (HA) surfaces, respectively. The HA surfaces were characterized with respect to wettability and roughness by means of water contact angles and atomic force microscopy, respectively. Both surface types were hydrophilic, and the average roughness (Sa) and surface enlargement (Sdr) were lower for the sintered compared to the spin-coated HA surfaces. The adsorbed amounts on the sintered HA increased as follows: HSA < laminin-1 < IgG < the protein mixture. For the competitive adsorption experiments, the adsorbed fractions increased accordingly: HSA < laminin-1 < IgG on both types of HA substratum. However, a higher relative amount of HSA and laminin-1 and a lower relative amount of IgG was found on the spin-coated surfaces compared to the sintered surfaces. The effects observed could be ascribed to differences in surface roughness and chemical composition between the two types of HA substratum, and could have an influence on selection of future implant surface coatings.

Keywords:

• dental implants
• protein mixture
• calcium phosphate
• nanotopography
• elutability
• ellipsometry
• gel electrophoresis

Acknowledgements

This study was supported by research grants from the Knowledge Foundation (KK stiftelsen, Biofilms – Research Centre for Biointerfaces), Malmö University and the Swedish Research Council. L. Lindh acknowledges financial support from the Swedish Laryng Foundation and T. Arnebrant from the Gustaf Th Ohlsson Foundation.