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Abstract
A bio-inspired durable anti-biofilm coating was developed for industrial stainless steel (SS) surfaces. Two polymers inspired from the adhesive and cross-linking properties of mussels were designed and assembled from aqueous solutions onto SS surfaces to afford durable coatings. Trypsin, a commercially available broad spectrum serine protease, was grafted as the final active layer of the coating. Its proteolytic activity after long immersion periods was demonstrated against several substrata, viz. a synthetic molecule, N-α-benzoyl-DL-arginine-p-nitroanilide hydrochloride (BAPNA), a protein, FTC-casein, and Gram-positive biofilm forming bacterium Staphylococcus epidermidis.
Acknowledgements
The research was partly supported by BELSPO (IUAP VI/27) and the Walloon Region (PPP program BIOCOAT). The authors thank the BIOCOAT team members for their contribution, in particular H. Vandegaardt, C. De Bona, A. Wislez and F. Farina. C. Detrembleur is Senior Research Associate of the F.R.S.-FNRS.