Abstract
The antimicrobial and anticaries effects of CaneCPI-5 were evaluated. Ninety bovine enamel samples were treated for 60 s with either phosphate-buffered-saline (PBS), 0.12% chlorhexidine (CHX), 0.05 mg ml−1 CaneCPI-5, 0.1 mg ml−1 CaneCPI-5 or 0.5 mg ml−1 CaneCPI-5. They were incubated with inoculum (human saliva + McBain's saliva) for the first 8 h. From then until the end of the experiment, the enamel was exposed to McBain saliva with sucrose and, once a day, for 5 days, they were treated with the solutions. At the end of the experimental period, resazurin and viable plate count assays were performed. Enamel demineralization was also measured. All concentrations of CaneCPI-5 and CHX significantly reduced the activity of biofilms compared with PBS. For viable plate counts, all treatments similarly reduced the lactobacilli and total streptococci; for the mutans streptococci, 0.05 mg ml−1 CaneCPI-5 performed better than CHX. All CaneCPI-5 concentrations significantly reduced the integrated mineral loss. This study represents the first step regarding the use of CaneCPI-5 within the concept of acquired enamel pellicle and biofilm engineering to prevent dental caries.
Acknowledgements
The authors thank FAPESP (2018/12041-7 and 2019/26070-1) and CNPq (407853/2018-9) for research grants awarded to the last author and FAPESP for the scholarship awarded to TTA (2019/08032-5) and PYTS (2017/16118-1). M.A.R.B is the recipient of a Research Productivity Scholarship from the National Council for Research and Development (CNPq 302371/2018-4).
Disclosure statement
The authors have declared no conflict of interest.
Authors contribution
Araujo TT and Buzalaf MAR conceived the experiments. Henrique-Silva F, Cruvinel T, Magalhães AC and Buzalaf MAR contributed to funding acquisition and methodology. Araujo TT, Camiloti GD, Valle AD, Carvalho TS, Câmara JVF and Shibao PYT conducted the experiments. Araujo TT, Valle AD, and Souza BM tabulated the data. Araujo TT, Valle AD, Souza BM, Silva NDG and Buzalaf MAR analyzed and interpreted the results. Araujo TT and Buzalaf MAR drafted the article. All authors reviewed and approved the manuscript.