Antimicrobial effects of surface pre-reacted glass-ionomer (S-PRG) eluate against oral microcosm biofilm

Abstract

This study investigated the antimicrobial activity of surface pre-reacted glass ionomer eluate (S-PRG) against oral microcosm biofilms collected from the oral cavity of patients. Dental biofilm samples were collected from three volunteers to form microcosm biofilms in vitro. Initially, screening tests were carried out to determine the biofilm treatment conditions with S-PRG eluate. The effects of a daily treatment for 5 min using three microcosm biofilms from different patients was then evaluated. For this, biofilms were formed on tooth enamel specimens for 120 h. Biofilms treated with 100% S-PRG for 5 min per day for 5 days showed a reduction in the number of total microorganisms, streptococci and mutans streptococci. SEM images confirmed a reduction in the biofilm after treatment. Furthermore, S-PRG also reduced lactic acid production. It was concluded that S-PRG eluate reduced the microbial load and lactic acid production in oral microcosm biofilms, reinforcing its promising use as a mouthwash agent.

Keywords:

• Microcosm biofilm
• Surface pre-reacted glass-ionomer
• Mouthwash
• Streptococci
• Streptococcus mutans

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work was supported by CNPq - National Council of Scientific and Technological Development (grant number 306330/2018-0) CAPES - Coordination for the Improvement of Higher Education Personnel (Scholarships)- and FAPESP 2018/08493-0; Fundação de Amparo à Pesquisa do Estado de São Paulo.