Effect of Acellular Amniotic Membrane Matrix Patch on Healing of Cut Surface After Sleeve Gastrectomy in Rats

ABSTRACT

Background: The aim of this study was to evaluate the effect of acellular amniotic membrane matrix patch on healing cut surface after sleeve gastrectomy in rats. Methods: Thirty male Wistar albino rats were divided into three groups: control (n = 10), Experiment-1 (n = 10), and Experiment-2 (n = 10) groups. Control group rats underwent only sleeve gastrectomy with primary gastrorrhaphy. Experiment-1 group rats underwent sleeve gastrectomy, primary gastrorrhaphy and covered with acellular amniotic membrane matrix patch. Experiment-2 group rats underwent sleeve gastrectomy, incomplete primary gastrorrhaphy and covered with acellular amniotic membrane matrix patch. All rats were sacrificed on the fifth postoperative day. Macroscopic findings and histopathologic alterations were evaluated and compared. Results: There was a statistically significant difference between levels of PMNs, granulation formation and vascularization, distributions of edema, type of mucosal epithelium, and fibroblastic proliferation according to groups (p < 0.05). The PMNs level in the Experiment-1 group was significantly lower than the control group (p < 0.05). In experiment groups, the level of granulation tissue, vascularization and fibroblastic proliferation was significantly higher than the control group (p < 0.05). The levels of edema and type of mucosal epithelium of the experiment groups were significantly lower than the control group (p < 0.05). Conclusion: Covering sleeve gastrectomy cut surface area with acellular amniotic membrane matrix results better healing. Moreover, acellular amniotic membrane matrix provides safe healing even in incomplete sutured area.

Keywords::

• morbid obesity
• sleeve gastrectomy
• acellular amniotic membrane matrix
• gastrorrhaphy
• rat
• stomach

This article is referred to by:

Preventing Leaks with a Patch: The Application of Amniotic Membrane Patches in an Animal Model in Sleeve Gastrectomies

DECLARATION OF INTEREST

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper. English Language usage has been revised and edited by Claire Olmez, BEd, MSc ELT.

ACKNOWLEDGMENTS

The author would like to acknowledge the contributions as follows. Study conception and design: S. Orman and S. Yol. Acquisition of data: S. Orman, S. Yol, H. Uzun, A. B. Ceyran, and F. Eyüboğlu. Analysis and interpretation of data: S. Orman, S. Yol, A. B. Ceyran, and F. Eyüboğlu. Drafting of manuscript: S. Orman, H. Uzun, and S. Yol. Critical revision: S. Orman and S. Yol.