Protective Effects of Hydrogen-Rich Saline on Experimental Intestinal Volvulus in Rats

Abstract

Background

Intestinal volvulus can cause morbidity and mortality. Surgical reduction, on the other hand, could result in ischemia-reperfusion (I/R) injury. Hydrogen rich saline solution (HRSS neutralizes free radicals in the body. This study aimed to investigate the effects of HRSS in I/R injury in experimental intestinal volvulus in rats.

Methods

Thirty rats were randomly allocated into 5 groups. All procedures were done under general anesthesia and sterile conditions in each animal. Five ml/kg of saline and HRSS were administered intraperitoneally (ip) in Sham (Group 1) and HRSS (Group 2) groups, respectively. Groups 3, 4, and 5 constituted the study groups in which volvulus was created in a 5-cm- long ileal segment 2 cm proximal to the ileocecal valve. After 2 hours the volvuli were reduced and following 2 hours of reperfusion, these segments were removed. In volvulus-I/R group (Group 3) no additional procedure was done. HRSS was administered shortly before reperfusion (reduction of the volvulus) in Treatment I (Group 4) and 1 h before experimental volvulus in Treatment II (Group 5) groups. Blood and intestinal tissue samples were obtained from all rats at the 4th hour. Both tissue and blood total oxidant (TOS) and antioxidant status (TAS) levels were determined and tissue histomorphologies were studied. Oxidative stress indices (TOS ÷ TAS) (OSI) were calculated.

Results

Tissue TOS and OSI levels and histomorphological injury scores were statistically lower in treatment groups than I/R group, whereas blood TOS and OSI levels were similar between the groups.

Conclusions

This study provides biochemical and histomorphological evidence that HRSS prevents intestinal damage in I/R injury caused by volvulus.

Keywords:

• Intestinal ischemia/reperfusion
• volvulus
• hydrogen-rich saline solution

Disclosure statement

No potential conflicts of interest was reported by the author(s).

Authors’ contributions

HO was involved in the experimental work, analysis, drafting and writing of the article; ZTwas conceived the idea for this project and involved in interpretation of data, revision of the work; RK was conceived the idea for this project and involved in interpretation of data, revision of the work; KS was involved in revision of the work and mentorship throughout; CK was involved in the experimental work and analysis; DD provided histological figures; CeK was involved biochemical analysis and data acquisition.