Allergenicity of main celery allergen rApi g1 and high-pressure treatment

Abstract

The structural changes and allergenicity of recombinant main celery allergen rApi g1 caused by the high pressure were studied. We have treated the buffer solutions of rApi g1 by high pressure at 500 MPa for pressure holding times 10 and 20 min and holding time temperatures 30 °C, 40 °C and 50 °C. The structural changes were studied by circular dichroismus (CD) spectra. The allergenic reaction of the rApi g1 was tested by Western blot analysis. The greatest changes of the structure were found at samples treated by 500 MPa at 50 °C. The samples treated at this temperature at pressure levels 400, 450 and 500 MPa and held for 10 and 20 min showed that protein structure changes are positively correlated with pressure. Western blot analysis evidenced that a pressure of 500 MPa held for 10–20 min at temperatures 30–50 °C did not change the allergenicity of the rApi g1 protein when compared with the untreated sample.

Keywords:

• celery
• Api g1
• allergen
• high pressure

Acknowledgements

The authors thank Dr Ondrej Julinek, Institute of Chemical Technology in Prague, for precise analysis of the structure changes by the ECD dichroismus method. Our work was supported by grant no. 2B06139