High-pressure treatment with silver carp (Hypophthalmichthys molitrix) protein and its allergic analysis

Abstract

The allergenicity and structural changes of silver carp allergens influenced by high-pressure treatment were studied. We treated the allergens at 100, 200 and 300 MPa for 10, 30 and 60 min at 20° C, used SDS–PAGE to separate the proteins and recognized the allergens by western blotting. Circular dichroism analysis was performed to characterize the structural change. From our study, we can determine that high-pressure treatment did not change the subunit composition, molecular weight or the allergenicity of silver carp allergens, but it did change the structure of the allergens.

Keywords:

• allergen
• western blot
• SDS–PAGE
• high-pressure treatment

Acknowledgements

The authors acknowledge the support from the Beijing University Health Science Center, Beijing, China, for providing the sera.