Abstract
The activation volume, Δ V ‡, of the solute incorporation process at the kink site of a glucose isomerase (GI) crystal was estimated using the pressure dependency of the step kinetic coefficient, βstep, and the three-dimensional nucleation rates J of GI crystals were analyzed using classical nucleation theory. Δ V ‡ took a negative value. The sticking parameter, s, for the addition of a GI tetramer to a critical nucleus drastically increased with increasing pressure. This was probably due to the decrease in the activation energy needed for the addition of the GI tetramer.
Acknowledgements
This work was partially supported by Grants-in-Aid (Nos. 16760014 and 19760009 (Y.S.)) for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.