Effect of hydrostatic pressure on the viability of non-adherent HL-60 cells

Abstract

We investigated the effect of hydrostatic pressure on the viability of non-adherent HL-60 cell line derived from leukemic cells over a high pressure range. The HL-60 cells are resistant to pressures of up to 100 MPa under pressurization for 20 min at 25°C. However, cell viability decreased markedly between 100 and 200 MPa, and almost all cells died above 200 MPa. In the case of pressures up to 25 MPa at 25°C for four days, the viability of HL-60 cells was inhibited by increasing the pressure above 20 MPa. Although high viability was observed between 1.6 and 2.0 MPa for adherent astrocytes, viability did not change over pressures up to 2.0 MPa in the case of non-adherent HL-60 cells. It is thought that the response of cells to pressure varies among cell types.

Keywords:

• non-adherent
• HL-60
• hydrostatic pressure
• viability
• trypan blue

Acknowledgement

This research was supported by the Ministry of Education, Culture, Sports and Technology Program for Strategic Research Foundation at Private Universities, 2010–2014.

Notes

^†This paper was presented at the 7th Conference on High Pressure Bioscience, Biotechnology (HPBB2012) at Otsu, Japan, 29 October–2 November 2012.