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High Pressure Research
An International Journal
Volume 44, 2024 - Issue 2
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Articles

Effect of incubation temperature after high hydrostatic pressure treatment of Escherichia coli on the detection of injured populations

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Pages 105-115 | Received 27 Feb 2024, Accepted 25 Mar 2024, Published online: 04 Apr 2024
 

ABSTRACT

Escherichia coli in phosphate-buffered saline was treated with high hydrostatic pressures (HHPs) of 400, 500, and 600 MPa at 25 °C for 10 min. The cell suspension was then plated on nonselective agar and incubated at 20, 25, 30, 35, 37, and 40 °C to study the effect of the incubation temperatures on the detection of HHP-injured populations. The number of detected cells was maximum when incubated at 25 °C for 72 h. Cold storage at 4 °C for 7 d after HHP-treatment improved the detection efficacies of incubations at 30–40 °C, implicating increased tolerance of HHP-injured cells against the mild heat. Combined incubation at 25 °C for more than 6 h with subsequent incubation at 35 °C shortened total incubation period from 72 h to 24 h while achieving the maximized colony number obtained by the incubation at 25 °C for 72 h.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work was supported by Ministry of Education, Culture, Sports, Science and Technology: [Grant Number 15K18762].

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