In vitro cytocompatibility evaluation of MGF-Ct24E chemically grafted and physically blended with maleic anhydride modified poly(D, L-lactic acid)

Abstract

As a growth repair factor, mechano-growth factor (MGF) and its 24 amino acid peptide analogs corresponding to the unique C-terminal E-domain (MGF-Ct24E) positively regulate bone regeneration. MGF-Ct24E was introduced into the poly(D, L-lactic acid) (PDLLA) to improve bone regeneration in our previous study. MGF-Ct24E-grafted PDLLA was chemically characterized and showed potential as a biofunctional polymer. In this study, we evaluated the cytocompatibility of MGF-Ct24E chemically grafted and physically blended with maleic anhydride modified PDLLA, relative to maleic anhydride modified PDLLA (MPLA) as the control. The surface properties of these three polymer films were characterized with scanning electron microscopy and X-ray photoelectron spectroscopy. Rat calvarial osteoblasts were seeded on the three polymer films, and cell adhesion, spreading, and proliferation were assessed with an inverted microscope, laser scanning confocal microscope, and a cell counting kit-8, respectively. The alkaline phosphatase activity and extracellular calcium production were exploited to characterize the differentiation and mineralization of rat calvarial osteoblasts on various polymer films. The results revealed that compared with MPLA, MGF-Ct24E-MPLA, and MGF-Ct24E/MPLA blends promoted adhesion, spreading, proliferation, and the later differentiation and mineralization process of rat calvarial osteoblasts. In addition, the positive effect of MGF-Ct24E-MPLA on rat calvarial osteoblasts was maintained longer than the MGF-Ct24E/MPLA blends. In conclusion, MGF-Ct24E-MPLA synthesized chemically might be a promising biomaterial for bone tissue engineering.

Keywords:

• poly(D, L-lactic acid)
• MGF-Ct24E
• blends
• cytocompatibility
• rat calvarial osteoblasts

Acknowledgments

This work was supported by a grant from the National Natural Science Foundation of China (11032012, 30870609), the Natural Science Foundation of CQ CSTC (2009BA4025, 2009BB4382, 2010BB5225).