Recoverability of freeze-dried doxorubicin-releasing chitosan embolic microspheres

Abstract

The purpose of this study is to investigate the recoverability of freeze-dried chitosan microspheres (MS). The factors influencing the integrity of chitosan MS during freeze-drying and rehydration procedures were determined, with focusing on choosing a suitable rehydration method and a freeze-drying excipient. Mean MS size, size distribution and sphericity of recovered chitosan MS were evaluated. Furthermore, the impacts of freeze-drying and rehydration procedure on the elasticity of chitosan MS were explored and the release profiles were evaluated. The recoverability of lyophilized chitosan MS was largely dependent on rehydration method and freeze-drying excipients. When using the optimized recovery processes, deformable drug-loaded chitosan MS can be rapidly recovered to exhibit the initial physico-mechanical properties such as elasticity. Release profiles also were not significantly changed after rehydration procedure. It is therefore expected drug-loaded chitosan MS can be stably freeze-dried with the prevention of drug release during storage and rapidly recovered to be used as deformable embolic materials possibly applicable for anti-cancer embolotherapy.

Keywords:

• chemoembolization
• elastic chitosan microsphere
• freeze-drying
• freeze-drying excipient
• doxorubicin
• recoverability

Acknowledgment

This work was supported by the Mid-career Researcher Program through NRF grant funded by the MEST (No. 2010-0027798).