Gene chip/PCR-array analysis of tissue response to 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer surfaces in a mouse subcutaneous transplantation system

Abstract

To evaluate the in vivo foreign body reaction to bio-inert 2-methacryloyloxyethyl phosphorylcholine (MPC) polymers, MPC polymer-coated porous substrates, with large surface area, were implanted subcutaneously in mice for 7 and 28 days, and the surrounding tissue response and cells infiltrating into the porous structure were evaluated. The MPC polymer surface induced low angiogenesis and thin encapsulation around the porous substrate, and slightly suppressed cell infiltration into the porous substrate. M1-type macrophage specific gene (CCR7) expression was suppressed by the MPC polymer surface after 7 days, resulting in the suppression of inflammatory cytokine/chemokine gene expression. However, the expression of these genes on the MPC polymer surface was higher than on the non-coated surface after 28 days. These findings suggest that MPC polymer surfaces successfully inhibit inflammatory responses during the early stage of tissue response, and seem to retard its occurrence over time.

Keywords:

• foreign body reaction
• poly(2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate)
• bio-inert
• porous substrate
• inflammation

Acknowledgments

This work was supported by CREST, Japan Science and Technology Agent (JST); the S-innovation Research Program for the ‘Development of biofunctional materials for realization of innovative medicine’, JST; and the JSPS Grant-in-Aid for Challenging Exploratory Research (Number 25560218).