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Abstract
Adipose derived stem cells (ADSCs) were cultured on collagen–silk fibroin films with microchannel and micropillar patterns to investigate the effects of cell morphology changes on osteogenic differentiation. Channel and pillar micropatterned films were prepared from collagen type I and silk fibroin. While higher ADSC proliferation profiles were obtained on micropillar blend film, microchannel blend films, however, caused twice higher aspect ratio and effective orientation of cells. Alkaline phosphatase activity of ADSCs was several times higher on microchannel surface when the measured activities were normalized to cell number. Effective deposition of collagen type I and mineral by the cells were observed for patterned and unpatterned films, and these extracellular matrix components were oriented along the axis of the microchannels. In conclusion, the use of collagen–fibroin blend film with microchannel topography increased the aspect ratio and alignment of cells significantly, and was also effective in the differentiation of ADSCs into osteogenic lineage
Acknowledgements
We acknowledge Prof. Dr Muharrem Demirogullari from Cag Hospital (Ankara, Turkey) for supplying the lipoaspirate material. This work was supported by METU through Grant BAP-07.02.2013.101. E.T. Baran acknowledges the Scientific and Technological Research Council of Turkey (TUBITAK) for the Post Doctoral 2232 BIDEP-TUBITAK Fellowship and the financial support from the METU Administration. We are grateful to Ministry of Development of Turkey for funding BIOMATEN through Grant DPT2011K120350.
Disclosure statement
No potential conflict of interest was reported by the authors.
Supplemental data
Supplemental data for this article can be accessed here http://dx.doi.10.1080/09205063.2015.1100494.