The Preventive Effect of Gentamicin in the Irrigating Solution on Endophthalmitis Caused by Methicillin-Resistant Staphylococcus epidermidis After Phacoemulsification with Intraocular Lens Implantation in Rabbits

ABSTRACT

Objective

To analyze the effect of gentamicin in the irrigating solution on endophthalmitis caused by methicillin-resistant Staphylococcus epidermidis after phacoemulsification with intraocular lens implantation.

Methods

Fifteen rabbits were randomly assigned into three groups. During surgery, group A was irrigated with gentamicin-free solution and injected with 100 μL of normal saline postoperatively, group B was irrigated with 80 μg/mL gentamicin and injected with 100 μl of MRSE suspension, group C was irrigated with gentamicin-free solution and injected with 100 μl of MRSE suspension. At different times, corneal endothelial cell count (CEC), inflammation grading，B-scan ultrasonography and histological examination were analyzed.

Results

No endophthalmitis occurred in groups A and B. Group C developed severe endophthalmitis, with massive inflammatory exudation in the vitreous cavity.

Conclusion

Irrigating solution containing gentamicin is favorable to reduce the incidence of MRSE endophthalmitis after phacoemulsification with IOL in rabbits.

KEYWORDS:

• Endophthalmitis
• gentamicin
• methicillin-resistant Staphylococcus epidermidis
• phacoemulsification with intraocular lens implantation

Disclosure statement

No potential conflict of interest was reported by the author(s).

Authors contributions

Ma W. designed this study and defined the intellectual content. Ma W., Hou G., and Wang J. acquired the data and analyzed the data. Liu T did the literature study. Liu T. and Tian F. acquired the experimental data. Ma W. drafted the paper and Tian F revised the paper. All authors have read and approved the final paper.

Ethics approval and consent to participate

Animal procedures were in accordance with the Guide for the Care and Use of Laboratory Animals and approved by the Ethics Committee of Tianjin Medical University Eye Hospital.

Data availability statement

The datasets used during the present study are available from the corresponding author upon reasonable request.

Additional information

Funding

The author(s) reported that there is no funding associated with the work featured in this article.