Effects of melatonin on plasma levels of TNF-α, IL-1 and IL-6 in mice after lipopolysaccharide administration

Abstract

Bidirectional dependence exists between the immune and neuroendocrine systems, with common chemical messengers, such as melatonin (MLT), having immunoregulatory functions in some mechanisms of endotoxic shock. We used 10 – 12-week-old female Balb/c mice, with an average weight of 22 – 24 g, maintained under a 12 h light – dark cycle. The LD₁₀₀ of lipopolysaccharide (LPS) was determined to be 10 mg/kg (ip.) from a dose – response curve. Pretreatment with MLT (10 mg/kg ip.) increased survival by 60%, decreased plasma TNF-αε and increased IL-1. No significant changes were observed in levels of plasma IL-6.

Keywords:

• Melatonin
• endotoxic shock
• TNF-α
• IL-1
• IL-6
• interleukins

Acknowledgements

The authors wish to thank to Veterinary Doctors Alberto Ramos M. and José Blanco F, and Fidencio Peña (Animal Supply: CIBO-IMSS. Guadalajara, Jalisco, México).