Morphologic changes in the pineal gland of rats exposed to continuous darkness

Abstract

We examined the pineal structure of rats exposed to constant darkness (DD) at light microscopic level. Two groups of rats were exposed to 12:12 light/dark cycle (LD) or DD from their prenatal ontogenesis and then for 3 months after birth. The gland structure of DD rats was observed to have an active appearance. Some of the observed pinealocytes with light nuclei from DD rats were determined to contain double nucleoli. Nuclear area and perimeter of both dark and light types were greater in rats kept in DD than in LD. Rats exposed to DD had more cells with light nuclei and lesser cells with dark ones than rats kept in LD. No significant differences in nuclear characteristics of intermediate type were found between rats kept in LD and those kept in DD. The activity of mammalian pineal can be altered by light conditions to which the animal is exposed.

Keywords:

• Constant darkness
• pinealocyte
• morphology

Acknowledgments

The authors are extremely grateful to our colleagues from Institute of Biology, Karelian Research Centre of Russian Academy of Sciences (Petrozavodsk, Russia), Andrey V. Bukalev and Anna N. Khmara (Petrozavodsk State University, Petrozavodsk, Russia) for the assistance for this study.