Inverse relationship between diurnal rhythms in plasma levels of thyroid hormones and pineal arylalkylamine-N-acetyltransferase (AANAT) activity in an air-breathing fish, Clarias gariepinus

Abstract

Plasma levels of thyroid hormones (T₄ and T₃) and pineal AANAT activity exhibited prominent diurnal rhythms during all the four phases (quiescent, progressive, breeding, and regressive) of the fish, Clarias gariepinus. During all the four phases, we observed that (i) the acrophase (peak) of the circadian rhythm of both T₃ and T₄ occurred around midday, while the acrophase of AANAT activity rhythm was recorded during midnight, (ii) a significant negative correlation existed between plasma levels of the thyroid hormones and AANAT activity, and (iii) a direct relationship existed between water temperature/daylength and plasma levels of thyroid hormones, and an inverse relationship between water temperature/daylength and AANAT activity. On the basis of these findings, we conclude that an inverse relationship exists between the diurnal rhythms in plasma levels of thyroid hormones and pineal AANAT activity. The observed inverse relationship between the levels of thyroid hormones and pineal AANAT activity seems to be maintained by seasonal changes in water temperature and daylength.

Keywords:

• Arylalkylamine N-acetyltransferase
• fish
• photoperiod
• pineal
• temperature
• thyroid

Acknowledgments

Financial assistance provided by the Department of Zoology, North-Eastern Hill University, Shillong under the UGC-SAP Programme is greatfully acknowledged.