ABSTRACT
In this study, effect high-energy diet with supplemented with copper and zinc on oxidative stress, leukocyte function and reproductive performance during the transition period in the crossbred cow under tropical settings. Advanced pregnant crossbred cows of two to four parity and lactation yield of >10 L/day were divided into three equal groups (n = 10 cow/group). Group I cows were fed basal diet to serve as control. Group II cows were supplemented with 20% additional concentrate, copper (15.7/kg DM) and zinc (22 mg/kg DM). Group III cows were provided with only energy in the form of 20% additional concentrate. Copper and zinc were supplemented from −4 before to +8 week of calving, whereas concentrate was given from +2 to +8 week after calving. Blood samples were collected at weekly interval from −4 to +8 week of calving. Group II had a significantly lower MDA, higher TAC and decreased activity of SOD and CAT than that of Group I (P < 0.05). Phagocytic activity and lymphocytic activity increased from 3 to 8 weeks postpartum (P < 0.05) in Group II and III indicating early restoration of leukocyte function. Resumption of postpartum oestrus was shorter in group II (P < 0.05).
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Acknowledgments
The authors thank The Director, Indian Veterinary Research Institute (ICAR-IVRI) for facilitating the work.
Disclosure statement
No potential conflict of interest was reported by the authors.
Authors’ contributions
BB carried out animal experimentation. NK and MG participated in the statistical analysis and assisted in preparation of the draft. SM, VPM and GS designed the work whereas BB and DJ prepared the manuscript. TV, AB, RM, AK and HK conceived and participated in the coordination of the study. All authors read and approved the final manuscript.