Single-layer graphene-based surface plasmon resonance sensor with dynamic evanescent field enhancement for biomarker study

ABSTRACT

In this work, we have developed a kind of single-layer graphene-based surface plasmon resonance (SLG-SPR) biosensor to detect C-reactive protein (CRP) and Prostate-specific antigen (PSA). In the experiment of testing CPR, the results obtained revealed that the changes in resonance wavelength of SLG-SPR biosensors are higher than that of the gold-film based SPR (Au-SPR) biosensors. Moreover, for the experiment of testing PSA, due to the dynamic evanescent field enhancement produced by a strong electric field coupling between the localized SPR (LSPR) of AuNPs and SPR of single-layer graphene-based film (SLG-film) that further amplify the evanescent field signal. We verified the SLG-SPR biosensors exhibited higher sensitivity than the Au-SPR biosensors and the SLG-SPR biosensor exceeded the traditional biosensor detection limit. Accordingly, the SLG-SPR biosensor based on dynamic optical enhancement can realize high sensitivity detection of low concentration biomarkers and can be applied to most of the trace biomarkers in theory.

KEYWORDS:

• Surface plasmon resonance
• localized surface plasmon resonance
• single-layer graphene
• gold nanoparticles
• sandwich immunoassay
• biomarker

Additional information

Funding

This work was supported by the Fundamental Research Funds for the Central Universities (2020JBM018) and the National Natural Science Foundation of China (No.61401019, No.61931018).