Abstract
In digital in-line particle holography (DIPH), minimum intensity projection (MIP) is a crucial step in the reconstruction procedure, the MIP image quality is significantly degraded by nonuniform-illumination, speckle noise and the artifacts from off-focal planes. Here, we report a method that improves the MIP image quality by fully exploiting the advantages of SNR-enhanced holography, along with a nouniform illumination correction procedure without requiring a-priori assumptions about particle structures. Experimental results for nonspherical particles show that the proposed method can correct nonuniform-illumination and mitigate the artifacts from off-focal planes, allowing one to extract the particle’s size, shape and transverse location from a 2D single image.
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Disclosure statement
No potential conflict of interest was reported by the author(s).