Differential expression of visfatin, leptin, stromal cell derived factor-1α, endothelial nitric oxide synthase, and vascular endothelial growth factor in human leiomyomas

Abstract

Aim: This study was aimed to understand expressions of the visfatin, leptin, stromal cell derived factor (SDF)-1α, endothelial nitric oxide synthase (eNOS), and vascular endothelial growth factor (VEGF) in human uterine leiomyomas (UL) and normal myometrium.

Method: This study investigated expression of visfatin, leptin, SDF-1α, eNOS and VEGF in 23 uterine leiomyoma patients and 10 normal myometrium by RT-PCR and western blot. Messenger RNA transcripts of SDF-1α, eNOS, VEGF and hypoxia inducible factor-1α (HIF-1α) were analyzed according to the size of UL by real-time PCR.

Results: There were no significant differences in expressions of visfatin and leptin between UL compared with normal myometrium. However, expressions of eNOS, SDF-1α and VEGF were significantly higher in both intramural and subserosal UL compared with normal myometrium. The expression of SDF1-α was significantly increased in small UL (<5 cm) compared to the large UL (≥5 cm), whereas the expressions of eNOS, VEGF and HIF-1α were higher in large UL than small UL.

Conclusions: This study shows that expression of SDF-1α, eNOS and VEGF were significantly higher in UL than myometrium with a different expression pattern according to the size of UL. However, expressions of visfatin and leptin had no significant differences between the two groups.

Chinese abstract

目的 这项研究的目的是了解子宫肌瘤及正常子宫肌层中内脂素、瘦素、基质细胞衍生因子 ( SDF) -1α、内皮性一氧化氮合酶 ( eNOS) 和血管内皮生长因子 ( VEGF) 的表达情况。

方法 应用逆转录-PCR以及蛋白免疫印迹法研究内脂素、瘦素、基质细胞衍生因子 ( SDF) -1α、内皮性一氧化氮合酶 ( eNOS) 和血管内皮生长因子 ( VEGF) 在23名子宫肌瘤患者和10名子宫正常的妇女的表达情况。根据子宫肌瘤的大小, 应用实时定量荧光PCR分析SDF-1α, eNOS、VEGF、缺氧诱导因子-1α ( HIF-1α) 信使RNA的转录情况。

结果 子宫肌瘤和正常子宫肌层中, 内脂素和瘦素的表达没有显著性差异。然而, 与正常子宫肌层相比, 不论是肌壁间肌瘤还是浆膜下肌瘤, 其SDF-1α, eNOS、VEGF的表达都是显著升高的。大肌瘤 ( ≥5cm) 相较于小肌瘤 ( <5cm) , 其eNOS、VEGF、HIF-1α的表达显著增加, 而SDF-1α的表达降低。

结论 本研究表明, 子宫肌瘤中SDF-1α, eNOS、VEGF的表达比正常子宫肌层显著增加, 同时, 根据子宫肌瘤的大小, 表达模式还有所不同。然而, 子宫肌瘤与正常子宫肌层中内脂素和瘦素的表达没有显著性差异。

Keywords:

• eNOS
• letpin
• SDF-1α
• uterine leiomyomas
• visfatin

Acknowledgements

This study was supported by Medical Research Institute Grant (2014-4), Pusan National University Hospital.

Declaration of interest

None of the authors have direct or indirect conflict interest associated with publishing the article.