Abstract
The aim of this study is to evaluate the impact of ovarian reserve and age of women on early morphokinetic parameters of embryos with a time-lapse monitoring system. In total, 197 infertile couples with poor ovarian reserve (Group 1, n = 41), normal ovarian reserve (Group 2, n = 59), or polycystic ovaries (Group 3, n = 97) were included. The time from insemination to the following events were analyzed: pronuclear fading (Pnf) and cleavage to 2, 3, 4 and 5 cells. The optimal ranges for morphokinetic parameters of t5, s2 and cc2 in each group were also evaluated. In total, 1144 embryos were evaluated. Morphokinetic parameters did not differ statistically between the groups. Data were analyzed according to different age groups (20–30, 30–40, >40). The morphokinetic parameters did not differ statistically in Group 1 and 3. In Group 2, the times from insemination to tPnf, t2, t3, t4 were significantly shorter in the younger age group than the older age group (p < 0.05). The percentages of optimal embryos, according to t5, s2 and cc2, did not differ statistically between the groups. In conclusion, ovarian reserve did not seem to affect the morphokinetic parameters of embryos.
Chinese abstract
本研究的目的是用延时检测系统评估不同年龄与不同卵巢储备功能的女性对早期胚胎的形态动力学参数的影响。共有197对不孕夫妇, 其中卵巢储备功能低下组(第1组, n = 41), 正常卵巢储备功能组(第2组, n = 59), 或多囊卵巢综合征组(第3组, n = 97)。从受精开始到原核消失(Pnf) 卵裂为2, 3, 4和5个细胞进行评估。每组形态动力学参数最佳范围是t5, s2 与 cc2。共对1144个胚胎进行了评估。各组之间形态动力学参数没有显著差异。根据不同年龄组(20 - 30, 30 - 40, >40)进行数据分析。组1和组3之间形态动力学参数没有统计学差异。在组2, 从受精到tPnf, t2, t3, t4的时间段, 年龄小的组比年龄大的组明显缩短(P<0.05)。根据t5, s2 和 cc2, 最佳胚胎的百分比, 各组之间没有显著差异。总而言之, 卵巢储备似乎并不影响胚胎的形态动力学参数。
Declaration of interest
The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this article.