Abstract
Objectives: This study aimed to investigate the Th1/Th2 cells in peripheral blood of PCOS patients, and assess the potential correlation between Th1/Th2 imbalance and obesity.
Methods: Thirty-nine PCOS patients and 23 age-matched controls were enrolled. The PBMCs were obtained before pharmacological intervention in women with or without PCOS. The profiles of Th1 (IFN-γ) and Th2 (IL-4) cytokines of CD3+CD− T lymphocyte subsets were analyzed by flow cytometry. Plasma sex hormones including E2, T, FSH, LH, and FINS, FPG were measured, together with BMI, WC, LH/FSH, E2/T and HOMA-IR index being calculated. Association between Th1/Th2 imbalance and BMI, WC were evaluated.
Results: The proportion of Th1 cells and Th1/Th2 ratio were significantly higher in PCOS patients than those in controls, accompanied by elevated T, LH, LH/FSH, FINS, HOMA-IR index and reduced E2/T. The Th1/Th2 ratio was increased when BMI and WC were enhanced in PCOS. Moreover, the significant difference of Th1/Th2 ratio was observed between WC subgroups of PCOS.
Conclusions: It is concluded that Th1 type immunity is predominant in systemic immunization of PCOS patients. Th1/Th2 immune imbalance is connected with obesity, especially abdominal obesity, and may be one of the underlying mechanism for the pathogenesis of PCOS.
Chinese abstract
伴或不伴多囊卵巢综合征的女性中Th1/Th2免疫失衡与肥胖之间的相关性研究
摘要
目的:本研究旨在观察PCOS患者外周血中Th1/Th2细胞的表达情况, 评估Th1/Th2失衡与肥胖之间的潜在相关性。
方法:本研究共纳入39例PCOS患者和23例年龄相匹配的正常对照者。在伴或不伴PCOS的女性进行药物干预之前提取PBMCs。通过流式细胞术分析CD3?CD?T淋巴细胞亚群的Th1 (IFN-γ)和Th2 (IL-4)细胞因子的分布。检测血浆性激素包括E2、T、FSH、LH以及FINS、FPG、BMI、WC、LH/FSH、E2/T, 并计算HOMA-IR指数。评估Th1/Th2失衡与BMI和WC之间的相关性。
结果:PCOS患者Th1细胞比例和Th1/Th2比值显著高于对照组, 伴有T、LH、LH/FSH、FINS和HOMA-IR指数升高以及E2/T降低。在PCOS患者中, BMI和WC升高时, Th1/Th2比值增加。此外, 还观察到在PCOS患者的WC亚组中, Th1/Th2比值存在显著差异。
结论:Th1型免疫在PCOS患者的全身免疫中占主导地位。 Th1/Th2免疫失衡与肥胖有关, 尤其是腹部肥胖, 这可能是PCOS患者潜在发病机制之一。
关键词: 多囊卵巢综合征, Th1/Th2免疫失衡, 肥胖, 体重指数, 腰围
Disclosure statement
The authors report no conflicts of interest.