The beneficial effects of nerolidol and hesperidin on surgically induced endometriosis in a rat model^*

Abstract

The objective of this article is to analyze the effects of nerolidol and hesperidin treatment on surgically induced endometriosis in a rat model. Endometriosis was induced in 24 healthy adult female Wistar albino rats via homologous uterine horn transplantation. Three operations were performed on each rat. After the second operation, the rats were randomized into control, nerolidol, and hesperidin treatment groups, and medications were administered for 2 weeks. The effects of the drugs on the endometriotic foci were evaluated after the third operation. Compared with the endometriosis control group, the average volume of the lesions was significantly lower in rats treated with hesperidin and nerolidol. Malondialdehyde levels were significantly reduced in the nerolidol-treated group, and glutathione levels and superoxide dismutase activity were significantly elevated in the endometriotic foci of both the hesperidin- and nerolidol-treated groups compared with the endometriosis group. Hesperidin and nerolidol treatment also improved histological parameters, such as hemorrhage, vascular congestion, necrosis, and inflammatory cell infiltration in the endometriotic foci. The results of this study demonstrated that treatment with the potent antioxidants nerolidol and hesperidin caused a significant regression of surgically induced endometriotic foci in rats.

摘要

本文的目的是在小鼠模型中分析橙花叔醇和橙皮苷治疗对手术后子宫内膜异位症的影响。通过同源子宫角移植在24只健康成年雌性Wistar白化鼠中诱发子宫内膜异位症。对每只小鼠进行三次手术。在第二次手术后, 将小鼠随机分为对照组, 橙花叔醇组和橙皮苷治疗组, 并给予药物治疗2周。在第三次手术后评估药物对子宫内膜异位灶的影响。与子宫内膜异位症组相比, 橙皮苷和橙花叔醇治疗组小鼠的病灶平均体积显著降低。在橙花叔醇处理组中, 丙二醛水平显著降低, 并且与子宫内膜异位症组相比, 橙花叔醇组和橙皮苷治疗组中子宫内膜异位灶中谷胱甘肽水平和超氧化物歧化酶活性显著升高。橙皮苷和橙花叔醇治疗也改善了子宫内膜异位病灶的组织学参数, 如出血, 血管充血, 坏死和炎性细胞浸润。该研究结果表明, 小鼠体内强效抗氧化剂橙花叔醇和橙皮苷能使治疗术后子宫内膜异位灶显著消退。

Keywords:

• Anti-inflammatory agents; endometriosis
• hesperidin
• nerolidol
• oxidative stress