Impacts of macrophage colony-stimulating factor (M-CSF) on the expression of natriuretic peptide precursor type C (NPPC) and regulation of meiotic resumption

Abstract

In mammalian follicles, oocytes are arrested at the diplotene stage of prophase I until meiotic resumption following the LH surge. C-type natriuretic peptide (CNP), encoded by natriuretic peptide precursor type C (NPPC), was found to be reduced by the LH surge in the follicle, and then lead to meiotic resumption by decreasing the level of cAMP in the oocyte. As a wide-spread cytokine, macrophage colony-stimulating factor (M-CSF) takes part in the oocyte development to maturation and ovulation. Our study describes the expression curve of M-CSF and its receptor and investigates the impact on the levels of CNP/NPPC to explore the possible mechanism for meiotic resumption in both vivo and vitro. The result shows after the LH/HCG surge, the expressions of M-CSF and its receptors decline significantly inside ovarian follicles, thus leading to transduction of a range of signals. Consequently, the expression of CNP reaches the peak at 2 h and immediately declines to a relatively low level.

摘要：

在哺乳动物的卵泡中, 卵母细胞在有丝分裂前期I的二倍体阶段被抑制, 直到在黄体生成素激增时减数分裂再次恢复。C型利钠肽（CNP）是由C型利钠肽前体（NPPC）编码的, 在卵泡中黄体生成素的激增降低了C型利钠肽（CNP）, 致使卵母细胞中cAMP的水平降低, 从而导致了减数分裂的恢复。巨噬细胞集落刺激因子（M-CSF）作为一种体内广泛分布的细胞因子, 参与了卵母细胞发育成熟和排卵过程。我们的研究描述了M-CSF及其受体的表达曲线, 并研究了其对CNP/NPPC水平的影响, 以探索在体内外减数分裂恢复的可能机制。结果表明, 在黄体生成素/人绒毛膜促性腺激素激增后, M-CSF及其受体在卵泡内的表达显著下降, 从而导致一系列信号的转导。因此, CNP的表达在2h达到峰值后, 并立即下降到一个相对较低的水平

The Chinese abstracts are translated by Prof. Dr. Xiangyan Ruan and her team: Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China.

Keywords:

• M-CSF
• M-CSFR
• NPPC
• CNP
• meiotic resumption

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This study was supported by the National Natural Science Foundation of China [31470078].