Transmembrane G protein-coupled receptor 30 gene polymorphisms and uterine adenomyosis in Korean women

Abstract

To compare the genetic distributions of 14G protein-coupled receptor 30 (GPR30) single-nucleotide polymorphisms (SNPs) between women with and without uterine adenomyosis. The study population comprised 69 Korean women. Uterine tissues from the adenomyosis and non-adenomyosis groups were used for DNA extraction. Pre-designed PCR/Sanger or Sequencing Primer and TaqMan^® SNP Genotyping Assays were used for the SNP genotyping of the GPR30 gene. Immunohistochemical staining was performed to confirm the GPR30 expression. Differences in genotype and allele frequencies between the two groups were calculated using Fisher’s exact test. The rs3802141 CT genotype was more common in the control group (p = .02), and the rs4266553 CC genotype was more common in the adenomyosis group (p = .02). The C allele of the SNP rs4266553 was more common in the adenomyosis group (p = .02). GPR30 expression was confirmed in 69 individuals in both groups. GPR30 gene polymorphism is presumed to affect the risk of adenomyosis with limited sample size. Further large-scale study is needed to explain the genetic influence of GPR30 gene polymorphism.

摘要

比较子宫腺肌病患者和非子宫腺肌病患者中, 14G蛋白偶联受体30 (GPR30)单核苷酸多态性(SNPs)的遗传分布。研究对象包括69名韩国女性。从子宫腺肌病患者组和非子宫腺肌病患者组中的子宫组织中提取DNA。采用预设计的聚合酶链反应/桑格或测序引物和TaqMan SNP基因分型分析用于GPR30基因的SNP基因分型分析。免疫组织化学染色证实GPR30的表达。两组间基因型和等位基因频率的差异采用Fisher’s精确检验。rs3802141 CT基因型在对照组(p = .02)更常见, 而rs4266553 CC基因型在子宫腺肌病组(p = .02)更常见。SNP rs4266553的c等位基因在子宫腺肌病组更常见(p = .02)。GPR30的表达在两组中的69名个体中得到证实。GPR30基因多态性可能影响了样本量有限的子宫腺肌病的发病风险。还需要进一步的大规模研究来解释GPR30基因多态性的遗传影响。

The Chinese abstracts are translated by Prof. Dr. Xiangyan Ruan and her team: Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China.

Keywords:

• Adenomyosis
• G protein-coupled receptor 30
• single-nucleotide polymorphism

Acknowledgements

All materials were obtained with informed consent under IRB-approved protocols.

Disclosure statement

The authors have no commercial, proprietary, or financial interests in the products or companies described in this article. No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by a Biomedical Research Institute grant from Kyungpook National University Hospital (2014) (grant no. general-14–05).