Serum and peritoneal fluid levels of interleukin-6 and interleukin-37 as biomarkers for endometriosis

Abstract

This study aimed to compare the levels of interleukin (IL)-1β, IL-6, IL-10, and IL-37 in the serum and peritoneal fluid of women with and without endometriosis. In addition, it aimed to determine the diagnostic values of the cytokines with significantly different concentrations. The levels of IL-1β, IL-6, IL-10, and IL-37 in the serum and peritoneal fluid samples of 40 women with endometriosis and 32 women without endometriosis were measured using an enzyme-linked immunosorbent assay. The serum and peritoneal fluid levels of IL-1β and IL-10 were not statistically significantly different between the endometriosis and control groups. The IL-6 and IL-37 levels in the serum and peritoneal fluid were higher in the endometriosis group than in the control group, and they were correlated with the stage of endometriosis. The AUC for the IL-37 was 0.897 for the serum and 0.934 for the peritoneal fluid, while the AUC for the IL-6 was 0.905 for the serum and 0.952 for the peritoneal fluid. Our results suggest that the serum and peritoneal fluid IL-6 and IL-37 levels were significantly increased in the endometriosis patients, indicating that these cytokines may serve as biomarkers for the diagnosis of endometriosis.

摘要

本研究旨在比较子宫内膜异位症患者及非子宫内膜异位症者血清和腹腔液中白细胞介素-1β、IL-6、IL-10和IL-37的水平。此外, 本研究旨在确定不同浓度细胞因子的诊断价值。采用酶联免疫吸附法测定40例子宫内膜异位症患者和32例非子宫内膜异位症患者血清和腹腔液中IL-1 β、IL-6、IL-10和IL-37水平。子宫内膜异位症组与对照组血清、腹腔液中IL-1 β、IL-10水平差异无统计学意义。子宫内膜异位症组血清和腹腔液中IL-6和IL-37水平均高于对照组, 且与子宫内膜异位症的分期有关。血清IL-37的AUC为0.897, 腹腔液的AUC为0.934；血清IL-6的AUC为0.905, 腹腔液为0.952。我们的结果提示子宫内膜异位症患者血清和腹腔液IL-6和IL-37水平显著升高, 提示这些细胞因子可能作为诊断子宫内膜异位症的生物标志物。

The Chinese abstracts are translated by Prof. Dr. Xiangyan Ruan and her team: Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China.

Keywords:

• Endometriosis
• interleukin-6
• interleukin-37
• biomarker

Disclosure statement

The authors report no conflicts of interests.

Additional information

Funding

This article was supported by the National Natural Science Foundation of China (No. 81801422).