Stress adaptation disorders play a role in rat gestational diabetes with oxidative stress and glucose transporter-4 expression

Abstract

Stress adaptation disorder exists in gestational diabetes mellitus (GDM) women, this study was to investigate the impact of stress adaptation disorder on glucose disposal and skeletal muscle glucose transporter4 (GLUT4) expression in GDM rat model. Rats were assigned randomly to Normal control (NC) group and GDM group. We analyzed the levels of corticosterone, epinephrine (E), norepinephrine (NE), malondialdehyde (MDA) and superoxide dismutase (SOD), interleukin-6 (IL-6) and expression of GLUT4 were also detected. Homeostasis model assessment (HOMA-IR) was used to evaluate insulin resistance. Compared with NC group, E, NE and Corticosterone were increased significantly, SOD and MDA were higher and GLUT4 expression was significantly lower in GDM rats. Corticosterone was positively related to MDA, MDA was positively and SOD was negatively related to HOMA-IR in both groups, IL-6 showed significant positive correlations with HOMA-IR. NE and Corticosterone were negative related to GLUT4 in GDM group. Stress hormones (E, NE and Corticosterone), MDA and IL-6 were the risk factors of GDM, SOD was the protective factor of GDM. Changes of stress hormones indicate that stress adaptation disorder exists in GDM rats. Stress adaptation disorder increase oxidative stress injury and inflammation, decrease GLUT4 and lead to incline of glucose uptake, result in hyperglycemia. Gaining an insight into correlations of these changes may be beneficial to maternal and child health and is important for the prevention of glycemia-related diseases.

摘要

妊娠期糖尿病（GDM）女性存在应激适应障碍, 本研究旨在探讨应激适应障碍对GDM大鼠模型糖代谢和骨骼肌葡萄糖转运蛋白4（GLUT4）表达的影响。将大鼠随机分为正常对照组（NC）和GDM组。测定皮质酮、肾上腺素（E）、去甲肾上腺素（NE）、丙二醛（MDA）、超氧化物歧化酶（SOD）、白细胞介素-6（IL-6）及GLUT4的表达。采用稳态模型评估（HOMA-IR）评估胰岛素抵抗。与NC组比较, GDM大鼠E、NE、皮质酮显著升高, SOD、MDA显著升高, GLUT4表达显著降低。皮质酮与MDA呈正相关, MDA与HOMA-IR呈正相关, SOD与HOMA-IR呈负相关, IL-6与HOMA-IR呈显著正相关。GDM组NE、皮质酮与GLUT4呈负相关, 应激激素（E、NE、皮质酮）、MDA、IL-6是GDM的危险因素, SOD是GDM的保护因素。应激激素的变化提示GDM大鼠存在应激适应障碍。应激适应障碍增加氧化应激损伤和炎症, 降低GLUT4, 导致葡萄糖摄取倾向, 导致高血糖。了解这些变化之间的相互关系可能对母婴健康有益, 对预防高血糖相关疾病也很重要。

The Chinese abstracts are translated by Prof. Dr. Xiangyan Ruan and her team: Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China.

Keywords:

• Stress adaptation disorder
• Oxidative stress
• Glucose transporter-4
• HOMA-IR

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by grants from the Foundation of Yuhuangding Hospital [grant no. 201809].