Effect of Bushen Huoxue recipe on women with thin endometrial ovulation disorder and a rat model of thin endometrium resulted from kidney deficiency-related blood stasis

Abstract

To examine the therapeutic effect of Bushen Huoxue recipe (BHR) on women with thin endometrial ovulation disorder and on a rat model of kidney deficiency-related blood stasis. A total of 60 women with thin endometrial ovulation disorder was enrolled. The primary outcome of the study was the pregnancy rate three menstrual cycles after treatment. The study also examined the changes in the type and thickness of uterine artery, uterine artery pulsatility index (PI) and endometrial resistance index (RI). To establish kidney deficiency-related blood stasis in Sprague Dawley (SD) rats, an intragastric administration of hydroxyurea and a tail vein injection of Dextran were given, following with a flashing of the uterine cavity with 95% anhydrous ethanol. A combined regimen of BHR and estradiol valerate significantly increased the rate of pregnancy in women with thin endometrial ovulation disorder. The treatment was accompanied by a significant increase in endometrial thickness and decreases in uterine artery PI and endometrial RI. In rats, kidney deficiency-related blood stasis caused severe loss in endometrial architecture, thickness, and numbers of gland and blood vessel compared to the healthy SD rats. Treatment with BHR could ameliorate the endometrial damages associated with kidney deficiency-related blood stasis.

摘要

为了观察补肾活血方（BHR）对薄型子宫内膜排卵障碍患者及肾虚血瘀大鼠模型的疗效。研究共纳入60例薄型子宫内膜排卵障碍患者。研究的主要结局是治疗三个月经周期后的妊娠率。研究还检测了子宫动脉类型和厚度、子宫动脉搏动指数(PI)和子宫内膜阻力指数(RI)的变化。为建立肾虚血瘀大鼠模型, 用羟基脲对SD大鼠进行灌胃并尾静脉注射右旋糖酐, 然后用95%无水乙醇冲洗宫腔。补肾活血方联合戊酸雌二醇可增加薄型子宫内膜患者的妊娠率。治疗后子宫内膜厚度显著增加, 子宫动脉PI和RI显著降低。与健康SD大鼠相比, 肾虚血瘀大鼠子宫内膜结构、厚度、腺体和血管数量显著减少。BSR治疗可改善肾虚血瘀导致的子宫内膜受损。

Keywords:

• Endometrium
• kidney deficiency-related blood stasis
• Bushen Huoxue recipe (BHR)

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This study was supported by Beijing Natural Science Foundation [No. 7164260].