Abstract
Introduction
Pregnant women with polycystic ovary syndrome (PCOS) are at increased risk of gestational diabetes (GDM). We aimed to assess the expressions of candidate microRNAs (miRs) in leukocytes of pregnant women with PCOS and GDM. Methods: Using real-time quantitative PCR method, miR-16-5p and miR-155-5p were examined from PCOS (n = 17), GDM (n = 14), GDM + PCOS (n = 11), and controls (n = 27). The relative expression levels of the candidate miRNAs were compared between patient and control samples. The results were calculated as relative quantification values (RQ). Results: After adjusting for potential confounding variables using ANCOVA, no significant differences were observed in miR-16-5p (p = .154) and miR-155-5p (p = .702) expressions among four groups. We found significantly upregulated miR-16-5p expression in PCOS patients (RQ = 12.97 ± 1.94; p = .0001), compared to controls (RQ = 2.32 ± 1.46). Decreased miR-155-5p was found in GDM women (RQ = 0.80 ± 0.36; p = .04), compared to controls (RQ = 1.78 ± 0.25). Body mass index had a positive correlation with 155-5p in the GDM group (r = 0.55; p = .038). We found strong positive correlation between 1-hour glucose and miR-155-5p in PCOS patients (r = 0.71; p = .001). Fasting glucose (r= −0.63, p = .03) presented significant inverse association with miR-16-5p in the GDM + PCOS group. Discussion: The present study shows for the first time that increased miR-16-5p expression is associated with PCOS in pregnancy. Moreover, downregulated miR-155-5p expression was found in relation with GDM.
摘要
前言:多囊卵巢综合症综合征(polycystic ovary syndrome, PCOS)孕妇患妊娠期糖尿病(gestational diabetes mellitus, GDM)的风险增加, 本研究旨在评估PCOS 和GDM 孕妇白细胞中候选microRNAs(miRs)的表达。
方法:采用实时定量PCR方法检测17例PCOS、14例GDM、11例GDM+PCOS和27例对照组的miR-16-5p和miR-155-5p。比较患者和对照样本中候选miRNAs的相对表达水平。结果以相对定量值(quantification values, RQ)计算。
结果:使用ANCOVA校正潜在的混杂变量后, 四组间miR-16-5p(p=0.154)和miR-155-5p(p=0.702)的表达无显著差异。与对照组(RQ=2.32±1.46)相比, PCOS患者miR-16-5p表达显著上调(RQ=12.97±1.94;p=0.0001)。与对照组(RQ=1.78±0.25)相比, GDM妇女的miR-155-5p降低(RQ=0.80±0.36;p=0.04)。GDM组的体重指数(body mass index, BMI)与155-5p呈正相关(r=0.55;p=0.038)。PCOS患者的1小时血糖和miR-155-5p之间有很强的正相关(r=0.71;p=0.001)。GDM+PCOS组空腹血糖(r=-0.63, p=.03)与miR-16-5p呈显著负相关。
结论:本研究首次表明miR-16-5p表达增加与妊娠PCOS有关。此外, miR-155-5p表达下调与GDM有关。
Disclosure statement
No potential conflict of interest was reported by the author(s).