Abstract
Objective
The main aim of this prospective study was to investigate the relationship between intrafollicular vitamin D and anti-Müllerian hormone (AMH) concentration and its impact on oocyte quality and developmental competence.
Methods
The analysis was performed on 208 follicular fluid (FF) samples obtained from 33 patients undergoing ovarian stimulation as part of in vitro fertilization (IVF) treatment that included intracytoplasmic sperm injection.
Results
Our study shows that vitamin D concentration in FF varies according to the developmental stage of the oocyte and corelates with embryo development status on day 3, while AMH concentration in FF is not correlated with the developmental potential of an oocyte. We demonstrated that the levels of vitamin D and AMH were higher in FF than in serum. Moreover we showed that AMH and vitamin D levels were positively correlated in FF but not in serum.
Conclusion
FF-AMH levels do not appear to be a suitable as noninvasive test of the developmental potential of an oocyte, while FF-vitamin D level can be used to evaluate whether embryos obtained from particular oocytes have potential of reaching the third day of culture. However, our results encourage further research to be carried out on a larger number of patients and testing additional components found in FF such as androgens.
患者卵泡液中的维生素D和抗苗勒管激素浓度 摘要
目的:本研究旨在探讨卵泡液内维生素D与抗苗勒管激素(AMH)浓度的关系及其对卵母细胞质量和发育能力的影响。
方法:对33例接受卵巢刺激(包括卵胞浆内精子注射)的体外受精(IVF)治疗的患者的208个卵泡液(FF)样本进行分析。
结果:我们的研究表明, FF中维生素D的浓度随卵母细胞的发育阶段而变化, 并与胚胎发育前3天的状态相关, 而FF中AMH的浓度与卵母细胞的发育潜能无关。我们发现FF中维生素D和AMH的水平高于血清。此外, 我们发现在FF中AMH与维生素D水平呈正相关, 而在血清中则无相关性。
结论:FF-AMH水平似乎不适合作为卵母细胞发育潜力的非侵入性检测, 而FF-维生素D水平可用于评估从特定卵母细胞获得的胚胎是否有潜力达到培养第三天。然而, 我们的研究结果需要更多的患者进行研究, 并测试FF中的其他成分, 如雄激素等。
Disclosure statement
No potential conflict of interest was reported by the author(s).