Abstract
Objective: Explored the expression of miR-29a in puerpera with RSA and its influencing mechanism. Method: 52 patients with RSA group were divided into 30 cases representing ≤3 abortions and 22 cases with ≥4 abortions,thirty healthy women who had induced abortion during the same period as the control group. The differences in the expression levels of miR-29a, FKBP52 mRNA, VEGF, MVD, and HIF-lα were compared between the groups by conducting a correlation analysis. Results: The expression levels of miR-29a, VEGF, MVD, and HIF-lα in the chorionic villus were significantly higher among patients in the group with ≥4 abortions than in those in the group with ≤3 abortions (P < 0.05), and the expression levels of FKBP52 mRNA were lower in the former than in the latter (P < 0.05). A Spearman correlation analysis revealed that the expression levels of miR-29a were positively correlated with the levels of VEGF, MVD, and HIF-lα (P < 0.05) and negatively correlated with the expression level of FKBP52 mRNA (P < 0.05). Conclusion: MiR-29a may be involved in the pathogenesis of RSA by inhibiting the protein expressions of FKBP52 and VEGF, promoting the apoptosis of trophoblasts, and impairing neovascularization, resulting in placental vascular dysplasia..
摘要
目的
探讨RSA产妇miR-29a的表达及其影响机制。
方法
将52例RSA患者分为30例≤3次流产和22例≥4次流产, 同期行人工流产的健康妇女30例作为对照组。通过进行相关性分析, 比较各组间miR-29a、FKBP52 mRNA、VEGF、MVD和HIF-1α 表达水平的差异。
结果
≥4次流产组绒毛组织中miR-29a、VEGF、MVD和HIF-1α的表达水平显著高于≤3次流产组(P<0.05), Spearman相关分析显示, miR-29a的表达水平与VEGF、MVD和HIF-1α的水平呈正相关(P<0.05), 与FKBP52 mRNA的表达水平呈负相关(P<0.01)。
结论
MiR-29a可能通过抑制FKBP52和VEGF的蛋白表达, 促进滋养层细胞凋亡, 抑制新生血管形成, 导致胎盘血管发育不良而参与RSA的发病机制。
Disclosure statement
No potential conflict of interest was reported by the authors.
Ethics approval and consent to participate
I confirm that I have read the Editorial Policy pages. This study was conducted with approval from the Ethics Committee of The Affiliated Hospital of Inner Mongolia Medical University (WZ(2022010)). This study was conducted in accordance with the declaration of Helsinki. Written informed consent was obtained from all participants.