ABO antigen levels on platelets of normal and variant ABO blood group individuals

Abstract

Although differential expression of ABO antigens on platelets is related to recipients’ platelet recovery and neonatal alloimmune thrombocytopenia in ABO-incompatible transfusion and pregnancy, the expression of ABO antigens on platelets in normal and variant ABO blood group individuals is rarely reported. Here, we analyzed the expression of ABO antigens on platelets in a cohort of 515 individuals with normal and variant ABO. The variants were also genotyped for ABO and/or FUT1 loci to distinguish inherited or acquired ABO variants. The results showed that ABO antigens expression on individual platelets was relatively stable during the storage period in vitro. The frequency of low-, moderate-, and high-expression phenotype in normal individual was 59.6%, 35.5%, and 4.9%, respectively. The platelets from para-Bombay and most inherited ABO subgroups express very low levels of ABO antigens, but the acquired ABO variants and the B₃ subgroup express normal or abnormally high levels of ABO antigens on platelets. The data extend the information of antigens expression on platelets, which may help to improve the strategy of platelets transfusion.

Keywords:

• ABO antigen
• platelet
• transfusion
• variant

Acknowledgment

The authors are grateful to MS Sunitha Vege of New York Blood Center for her advice and English revision.

Disclosure statement

No potential conflict of interest was reported by the author.

Additional information

Funding

The work was supported by the National Natural Science Research Foundation: [grant number 81570170], the Science Research Foundation of Zhejiang Province: [grant numbers 2013C33193, LGF19H080004, 2017C33085 and LY17H080003], the Medical Science Research Foundation of Zhejiang Province: [grant numbers WKJ-ZJ-1510 and 2016RCB006], and Zhejiang Provincial Program for the Cultivation of High-level Innovative Health Talents.