Peroxidase-labelling of Chicken Antibodies

Abstract

Chicken antibodies have several advantages over mammalian antibodies due to the phylogenetical differences between birds and mammals, resulting in an increased sensitivity and a decreased background in many immunological assays. So far, there has been a very limited use of avian antibodies. This is probably partly due to tradition but also because there are very few optimized methods for the labelling of chicken antibodies. We report upon the suitable conditions for peroxidase-labelling of chicken antibodies with a modified periodate method.

Keywords:

• Antibody
• Chickens
• Elisa
• Igg
• Periodate
• Peroxidase