Evaluation of cell viability, DNA single-strand breaks, and nitric oxide production in LPS-stimulated macrophage RAW264 exposed to a 50-Hz magnetic field

Abstract

Purpose: Synergistic effects between cellular oxidative stress and magnetic fields may explain the adverse biological effects of 50/60 Hz magnetic fields. To determine whether this hypothesis holds in macrophage RAW264 cells, we measured DNA single-strand breaks (SSB), cell viability, and nitric oxide (NO) production in cells with or without exposure to 0.5-mT, 50-Hz magnetic fields for 24 h and with or without simultaneous stimulation via the bacterial endotoxin, lipopolysaccharide (LPS).

Materials and methods: Macrophages stimulated with 10 ng/ml LPS for 1 h were exposed to or not exposed to a magnetic field and were then subjected to (1) the alkaline comet assay to measure SSBs, (2) trypan-blue exclusion assay for cell viability, and (3) measurements of NO for evaluation of oxidative stress.

Results: The 50-Hz magnetic field enhanced DNA SSB and decreased cell viability only in the LPS-stimulated macrophages in which NO production was greatly enhanced. The magnetic field alone did not alter NO production.

Conclusion: Co-stimulation of the cell with LPS and a 50-Hz magnetic field promoted SSB and lowered cell viability, but these were not mediated by LPS-induced NO production.

Keywords:

• Low frequency magnetic field (LFMF)
• oxidative stress
• single-strand breaks/double-strand breaks (SSB/DSB)

Acknowledgements

This work was supported by the Grant-In-Aid from MEXT (to HM; 426610128), and MN was supported by a scholarship (Weaving Science Web beyond Particle-Matter Hierarchy) from Tohoku University.

Disclosure statement

The authors report no conflicts of interest. The authors alone are response for the content and writing of the paper.