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Abstract
Purpose
To study the environmental radiation effects of wild animals after the Fukushima Dai-ichi nuclear power plant accident, we assessed effects on hematopoietic progenitor cells (HPCs) in large Japanese field mice (Apodemus speciosus).
Materials and methods
A. speciosus were collected from three contaminated sites and control area. The air dose-rates at the control and contaminated areas were 0.96 ± 0.05 μGy/d (Hirosaki), 14.4 ± 2.4 μGy/d (Tanashio), 208.8 ± 31.2 μGy/d (Ide), 470.4 ± 93.6 μGy/d (Omaru), respectively. We investigated possible DNA damage and pro-inflammatory markers in the bone marrow (BM) cells. The colony-forming potential of BM cells was estimated by the number of HPC colony-forming cells. Radiation-induced genomic instability (RIGI) in HPCs was also analyzed by quantifying delayed DNA damage in CFU-GM clones.
Results
Although no significant differences in DNA damage and inflammation markers in BM cells from control and contaminated areas, the number of HPC colonies exhibited an inverse correlation with air dose-rate. With regard to RIGI, no significant differences in DNA damage of CFU-GM clones between the mice from the control and the three contaminated areas.
Conclusions
Our study suggests that low dose-rate radiation of more than 200 Gy/d reduced HPCs, possibly eliminating genomically unstable HPCs.
Acknowledgments
The authors express their deep appreciation of Namie Town’s local government in Fukushima Prefecture, Japan. The authors thank the laboratory staff for technical and secretarial assistance. The authors would also like to thank Editage (www.editage.jp) for English language editing.
Disclosure statement
No potential conflict of interest was reported by the author(s).
Additional information
Funding
Notes on contributors
Kentaro Ariyoshi
Kentaro Ariyoshi, Ph.D., is an associate professor at the Integrated Center for Science and Humanities, Fukushima Medical University.
Tomisato Miura
Tomisato Miura, Ph.D., is an associate professor at Department of Bioscience and Laboratory Medicine, Hirosaki University Graduate School of Health Sciences.
Kosuke Kasai
Kosuke Kasai, Ph.D., is a senior lecturer at Department of Bioscience and Laboratory Medicine, Hirosaki University Graduate School of Health Sciences.
Valerie Swee Ting Goh
Valerie Swee Ting Goh, M.Sc., is a doctoral student at Department of Bioscience and Laboratory Medicine, Hirosaki University Graduate School of Health Sciences, and a post-graduate research scholar with the Singapore Nuclear Research and Safety Initiative (SNRSI).
Yohei Fujishima
Yohei Fujishima, Ph.D., is an assistant professor at Department of Radiation Biology, Tohoku University School of Medicine.
Akifumi Nakata
Akifumi Nakata, Ph.D., is an associate professor at Faculty of Pharmaceutical Science, Department of Pharmacy, Hokkaido University of Science.
Atsushi Takahashi
Atsushi Takahashi, Ph.D., is an assistant professor at Tohoku University Hospital.
Yoshinaka Shimizu
Yoshinaka Shimizu, Ph.D., is an assistant professor at Department of Oral Medicine and Surgery, Graduate School of Dentistry, Tohoku University.
Hisashi Shinoda
Hisashi Shinoda, Ph.D., is a professor emeritus at Graduate School of Dentistry, Tohoku University.
Hideaki Yamashiro
Hideaki Yamashiro, Ph.D., is an associate professor at Faculty of Agriculture, Niigata University
Colin Seymour
Colin Seymour, Ph.D., is a professor at Medical Physics & Applied Radiation Sciences, McMaster University.
Carmel Mothersill
Carmel Mothersill, Ph.D., is a professor at Department of Biology, McMaster University; Medical Physics & Applied Radiation Sciences, McMaster University.
Mitsuaki A. Yoshida
Mitsuaki A. Yoshida, Ph.D., is a professor in Hirosaki University Institute of Radiation Emergency Medicine Department of Radiation Biology.