Summary
DNA isolated from T7 phage or deposits of this phage were irradiated with various qualities of radiation, including track-segment irradiations by charged heavy particles. The amount of single- and double-strand breakage was determined by analysis of the sedimentation boundary in an analytical ultracentrifuge. The results show that
(1) a single-strand break is produced on average for each energy-loss event in the DNA molecule
(2) an energy-loss event has a chance of about 1 in 20 of producing a double-strand break, with little dependence on LET in the range up to 100 keV/μm
(3) there is no significant effect of oxygen on the production of either single-or double-strand breaks.