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Summary
Enzyme variations produced by local x-irradiation on epidermis have been studied by a microspectrophotometric technique.
Analysis of enzyme response in function of the post-irradiation time showed clear changes after 2 days using 16 000 rads. A consistent total enzyme content (TEC) increase was found in all irradiation conditions, for the four oxidative enzymes under study (SDH, LDH, G-6-phDH and NADP diaph). The mean enzyme concentration (MEC = TEC/epithelial thickness) increased for NADP diaph and G-6-phDH, while decreasing for LDH and SDH activities. These findings suggest a metabolic adjustment to other oxidative pathways.