Effects of Electrons and Neutrons on the Synthesis of RNA in Resistant and Sensitive Strains of the Slime-mould Dictyostelium Discoideum and the Modifying Effect of Caffeine

Summary

RNA synthesis was investigated after irradiation in resistant and sensitive lines of the slime-mould Dictyostelium Discoideum. When ³H adenine was used as a precursor to RNA, incorporation increased after irradiation in the resistant WT line but not in the sensitive line (γs-13). The extent of RNA synthesis after irradiation was correlated with the shoulder width on the survival curve of the resistant line. When this was reduced by irradiating with neutrons, or treatment with caffeine RNA synthesis was also reduced. No preferential synthesis of one RNA species occurred; there was increased labelling in all RNA species after irradiation. Sucrose gradient analysis of ribosomal RNA extracted from irradiated cells and free of messenger RNA revealed no apparent difference in composition from that extracted from unirradiated cells. Increased RNA synthesis after irradiation may form part of the recovery process in the resistant cells.