Effects of Some ·OH Scavengers on the Radiation Sensitization of Bacterial Spores by p-nitroacetophenone and O₂ in Suspension

Summary

Spores of Bacillus megaterium were irradiated in suspension with 50 kVp X-rays under three reference conditions: in anoxia (i.e., 100 per cent N₂); in anoxia with 2 mM p-nitroacetophenone (PNAP), a concentration that shows the maximum amount of sensitization by this agent; and in air. The responses were then measured when different concentrations of an hydroxyl radical scavenger were also present. Allyl alcohol, t-amyl alcohol, t-butanol, ethanol, glycerol, the formate ion, and methanol were the ·OH scavengers that were used. A comparison of the effects these additives have on the three reference responses provides indirect, comparative information on the sensitizing processes of PNAP and O₂.

Neither t-amyl alcohol nor t-butanol affects the response in air or N₂ alone. Both these additives, however, can reduce the sensitization from PNAP slightly. The other additives have significant protective effects under the three conditions. In general, the additives have similar effects in air and in 100 per cent N₂ but much greater protective effects in PNAP. Although the sensitizing mechanisms are not identified by these experiments, the effects of O₂ and PNAP, as judged by the desensitizing actions of these additives, appear quite different.