Summary
Protoplasts from a diploid Nicotiana species were used to investigate the effects of γ-rays and fast neutrons. The damage was assessed by scoring the relative plating efficiencies. The effects of single acute doses, as well as fractionated doses, are described for both qualities of radiation. The dose-r.b.e. relationship, expressing the effectiveness of fast neutrons relative to γ-rays as a function of dose, appears similar to that of most animal systems, with higher values in the region of lower doses. The fractionation of γ-ray doses, with sufficiently long time intervals between split doses, allowed a substantial recovery, as known also for X-rays. On the other hand, the fractionation of a fast neutron dose into low single fractions markedly increased the biological effectiveness of the total dose. This could not be explained by cell progression during resting time intervals and necessitated an additional hypothesis, possibly involving other mechanisms in the specific action of low doses of radiation.