Eluting Solution Composition Affects DNA Double-strand Break Analysis by Filter Elution

Summary

The filter elution technique was used to assay for ¹³⁷Cs-induced DNA double-strand breaks (dsb) in V79 Chinese hamster cells. The elutions were performed using two different sets of lysing and eluting solutions at pH 7·2 and 9·6. The data agree with those of others who have demonstrated differences in elution profiles at pH 7·2 and 9·6. In addition, the data indicate that solution composition has a significant effect on elution.