Cell Survival and DNA Double-strand Break Repair Following X-ray or Neutron Irradiation of V79 Cells

Summary

We have investigated the effects of 250 kVp X-rays and 2·3 MeV (mean energy) neutrons on the cell survival, DNA double-strand break (dsb) induction and repair (using the Kohn neutral elution technique) in V79 cells. The lethal effects of neutrons were shown to be significantly greater than for a similar dose of X-rays (RBE = 3·55) at 10 per cent survival). However, the RBE for dsb induction, in a dose range of 10–50 Gy, was 1.

On investigating the repair of DNA dsb induced by either X-ray or neutron irradiation, clear differences in the pattern of repair were found. Both a fast and a slow component of repair were seen in both cases; the former, however, was reduced following neutron irradiation and, since the amount of slow repair was similar in both cases, this resulted in proportionally more unrejoined breaks after neutrons.

These experiments were carried out with elution buffer at pH 9·6; however, when similar experiments were performed at pH 7·2 the results obtained support our earlier findings. We suggest that these differences in DNA dsb repair reflect basic differences in the nature of the lesions induced by high- and low-LET ionizing radiations.