Efficacy of yeasts in the management of root-knot nematode Meloidogyneincognita, in Flame Seedless grape vines and the consequent effect on the productivity of the vines

Abstract

Twenty-two yeast strains were screened for their efficacy in suppression of root-knot nematode Meloidogyne incognita on Flame Seedless grapevines. The most effective seven strains were chosen to study their effect on nematode population in vitro and in vivo as well as their impact on crop productivity and fruit quality. Data showed that all treatments significantly reduced the number of juveniles in vitro after 24 and 48 h. The highest percentage of nematode mortality was achieved by the application of Pichia gluilliermondii Moh10, Pachytrichospora transvaalensis Y-1240, Candida albicans Moh Y-5 and Geotichum terrestre Y 2162. Similarly, under greenhouse conditions, all treatments significantly reduced the number of juveniles and disease index compared to the untreated plants during both incubation periods (30 and 50 days). Under field conditions, the tested yeast strains as well as Nemacour (nematocide) resulted in a significant reduction (P<0.05) of populations of Meloidogyne incognita compared to the control in two successive seasons tested. The beneficial impact of application of yeast strains was reflected in crop productivity on Flame Seedless grapevines as well as fruit quality. Percentages of vegetative buds, flower buds, shoot length and leaf number were significantly enhanced as a result of application of the different yeast strains and Nemacour. Bunch weight, berry weight and berry numbers were significantly increased by Pachytrichospora transvaalensis Y-1240, Geotichum terrestre Y-2162 and Candida albicans Moh Y-5. Nemacour and other yeast strains did not show any significant differences compared to the infected vines. Percentage of total soluble solids (TSS%) increased by applying all yeast strains, whereas Nemacour treatment gave the highest value of both TSS percent and percentage of acidity and increased anthocyanins in the skin of berries.

Keywords:

• Meloidogyne
• grapevine
• biocontrol
• Candida
• Cryptococcus
• Geotichum
• Issatchenkia
• Pachytrichospora
• Pichia

Acknowledgements

The authors are deeply grateful to Professor J.L.F. Kock, Professor of Yeast Taxonomy and Biotechnology, Department of Microbiological, Biochemical and Food Biotechnology, Faculty of Natural and Agricultural Sciences, University of the Free State, South Africa, for providing the yeast strains (strain Nos. 12–22). The authors also thank the owner of the vineyard of Flame Seedless grapevines at Sahel Selem, Assiut Governorate, Egypt, for his permission to carry out the research and assistance.