Evaluation of Bacillus subtilis (JN032305) biofungicide to control chilli anthracnose in pot controlled conditions

Abstract

Colletotrichum gloeosporioides (Penz.) causing anthracnose is a potent pathogen of chilli resulting in significant yield loss. The in vitro root colonisation study showed an increase in root bacterial count by 10 × 10⁵ colony forming units/cm root for Bacillus subtilis after 15 days of germination. Population level of the antagonist was stable in talc till the 180th day (30°C – 1.6 × 10⁸; 4°C – 1.9 × 10⁸) and in lignite till the 150th day (30°C – 1.5 × 10⁸; 4°C – 1.3 × 10⁸). Combined application of B. subtilis and carbendazim enhanced all biometric parameters with reduction in disease incidence. Soil, seed, root dip and foliar spray treatment significantly enhanced the growth parameters of chilli in B. subtilis inoculated treatments in comparison to the untreated control. Seed application resulted in highest plant fresh weight (76.84 g) and dry weight (34.17 g) compared to the untreated control (50 g and 21 g fresh and dry weight, respectively). Highest plant height ranging from 70 cm (soil application) to 77 cm (dip treatment) with Bacillus inoculation was comparable with carbendazim treatment (61 cm with soil application) and 78 cm (dip treatment) and significantly higher than the untreated control (58.2 cm with soil and 61 cm with application, respectively). Dip treatment resulted in significant increase in root length with B. subtilis (33 cm) and carbendazim (32.5 cm) in comparison to untreated control (15 cm). Co-inoculation of pathogen with B. subtilis (singly and with carbendazim) not only reduced the disease incidence but also improved all the biometric parameters in comparison to challenge inoculation. Root dip application was effective in promoting growth while seed application was effective in disease control.

Keywords:

• Bacillus subtilis
• chilli pot studies
• biocontrol
• C. gloeosporioides
• disease incidence

Acknowledgements

Our sincere thanks are due to the Management of Jain University (Jain Group of Institutions) for all the necessary infrastructural support provided to carry out this work.

Disclosure statement

No potential conflict of interest was reported by the authors.