Microbial interference ameliorates essential oil yield and diminishes root-knot infestation in sweet basil under field conditions

Abstract

Despite the vast exploration of bioinoculants for disease resistance and crop yield enhancement, knowledge about their impact on quality and quantity of essential oil as well as induction of antioxidant is scarce, especially in medicinal and aromatic crops. In the present two-year field experimentation, a subset of Bacillus spp., namely Bacillus flexus, B. subtilis, B. megaterium and B. aryabhattai, was evaluated against the most devastating root-knot nematode, Meloidogyne incognita (Kofoid and White) Chitwood infestation and essential oil yield enhancement in Ocimum basilicum var. CIM-Soumya (Sweet basil). The plants treated with B. subtilis showed significant (P < 0.05) enhancement in fresh biomass (42%), essential oil content (121%), nutrient uptake (83%) and reduced root-knot infestation (54%) as compared to untreated control. The defence afforded by bioinoculants was observed based on augmentation of the phenolic (97%), flavonoid (93%), free radical scavenging activity (34%) and total antioxidant (33%) in Bacillus-treated infected plants as compared to untreated infected plants. The higher concentration of essential oil constituents, namely linalool (54%) and methyl chavicol (33%), was also recorded in same treatment. An increase in essential oil yield was reflected by induction of antioxidant machinery. The present study for the first time delineates nematicidal potentials of B. flexus and B. aryabhattai. Altogether these findings suggest that exploitation of bioinoculants could be helpful for effective management of the root-knot nematode infestation along with higher qualitative and quantitative essential oil yield with augmented antioxidants in sweet basil.

Keywords:

• antioxidant
• Bacillus spp.
• essential oil
• Meloidogyne incognita
• Ocimum basilicum
• root-knot index

Supplemental material

Supplemental data for this article can be accessed here: http://dx.doi.org/10.1080/09583157.2015.1036728.

Additional information

Funding

This research was funded by CSIR-Twelfth Five Year Plan Project [BSC0203]. The authors express their sincere gratitude to the Director, CSIR-CIMAP, Lucknow, India for providing necessary facilities and encouragement during the course of investigation.